biospin p30 column (Bio-Rad)
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biospin p30 column
Biospin P30 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+gel+p30/Bio-Spin+P-30+Gel+Columns/pm41887061-181-10-13
Average 95 stars, based on 149 article reviews
Biospin P30 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+gel+p30/Bio-Spin+P-30+Gel+Columns/pm41887061-181-10-13
Average 95 stars, based on 149 article reviews
biospin p30 column - by Bioz Stars,
2026-09
95/100 stars
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GPC Assay:Article Title: Sorption of benzene derivatives onto insolubilized humic acids Article Snippet: Humic substances resulting from the biological decomposition of organic matter account for the greatest part of the organic fraction in surface water, groundwater, and soil (Piccolo 2001, Leone et al. 2017).. In chemical terms, humic substances are polymers with broad molecular-mass distribution and high chemical heterogeneity, consisting of a skeleton of aromatic rings bearing quinone, phenol, and catechol functional groups and a number of substituents such as carboxyl, hydroxyl, and sugar moieties.. Humic acids (HA) are a fraction of humic substances characterized by a pronounced acidic character and solubility in water at neutral or basic pH (Allard 2006). Gel Permeation Chromatography:Article Title: Sorption of benzene derivatives onto insolubilized humic acids Article Snippet: Humic substances resulting from the biological decomposition of organic matter account for the greatest part of the organic fraction in surface water, groundwater, and soil (Piccolo 2001, Leone et al. 2017).. In chemical terms, humic substances are polymers with broad molecular-mass distribution and high chemical heterogeneity, consisting of a skeleton of aromatic rings bearing quinone, phenol, and catechol functional groups and a number of substituents such as carboxyl, hydroxyl, and sugar moieties.. Humic acids (HA) are a fraction of humic substances characterized by a pronounced acidic character and solubility in water at neutral or basic pH (Allard 2006). Incubation:Article Title: Evolutionary Emergence, Optimization, and Co-Option of Aminoacylation Ribozymes Article Snippet: .. Reactions were incubated at room temperature with rotation for 90 minutes and stopped by desalting using Micro Bio-Spin Columns with Bradford Protein Assay:Article Title: Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation Article Snippet: .. Buffer G (20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% glycerol) Buffer J (50 mM Tris-HCl, pH 7.9, 6 M guanidine-HCl, and 5% glycerol) Buffer TGEβ (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, and 5% glycerol) Buffer TGED (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 1 mM DTT, and 5% glycerol) Dimethylformamide (DMF) (Sigma-Aldrich) Bradford Protein Assay kit (BioRad) Spectra/Por 3 dialysis tubing (3.5 kDa molecular weight cutoff) (Spectrum Labs) Molecular Weight:Article Title: Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation Article Snippet: .. Buffer G (20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% glycerol) Buffer J (50 mM Tris-HCl, pH 7.9, 6 M guanidine-HCl, and 5% glycerol) Buffer TGEβ (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, and 5% glycerol) Buffer TGED (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 1 mM DTT, and 5% glycerol) Dimethylformamide (DMF) (Sigma-Aldrich) Bradford Protein Assay kit (BioRad) Spectra/Por 3 dialysis tubing (3.5 kDa molecular weight cutoff) (Spectrum Labs) Membrane:Article Title: Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation Article Snippet: .. Buffer G (20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% glycerol) Buffer J (50 mM Tris-HCl, pH 7.9, 6 M guanidine-HCl, and 5% glycerol) Buffer TGEβ (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, and 5% glycerol) Buffer TGED (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 1 mM DTT, and 5% glycerol) Dimethylformamide (DMF) (Sigma-Aldrich) Bradford Protein Assay kit (BioRad) Spectra/Por 3 dialysis tubing (3.5 kDa molecular weight cutoff) (Spectrum Labs) Chromatography:Article Title: Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation Article Snippet: .. Buffer G (20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% glycerol) Buffer J (50 mM Tris-HCl, pH 7.9, 6 M guanidine-HCl, and 5% glycerol) Buffer TGEβ (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, and 5% glycerol) Buffer TGED (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 1 mM DTT, and 5% glycerol) Dimethylformamide (DMF) (Sigma-Aldrich) Bradford Protein Assay kit (BioRad) Spectra/Por 3 dialysis tubing (3.5 kDa molecular weight cutoff) (Spectrum Labs) Spectrophotometry:Article Title: Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation Article Snippet: .. Buffer G (20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% glycerol) Buffer J (50 mM Tris-HCl, pH 7.9, 6 M guanidine-HCl, and 5% glycerol) Buffer TGEβ (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, and 5% glycerol) Buffer TGED (20 mM Tris-HCl, pH 7.9, 0.1 mM EDTA, 1 mM DTT, and 5% glycerol) Dimethylformamide (DMF) (Sigma-Aldrich) Bradford Protein Assay kit (BioRad) Spectra/Por 3 dialysis tubing (3.5 kDa molecular weight cutoff) (Spectrum Labs) Labeling:Article Title: m 6 A Facilitates eIF4F-Independent mRNA Translation Article Snippet: The capped or non-capped mRNA (Hsp70-5'UTR) was synthesized using the mMessage mMachine T7 Ultra kit (Ambion). .. The probe was labeled in a 50ul reaction mixture containing 2 μl RNA probe (1 μM), 5 μl 10×T4 PNK buffer (NEB), 1 μl T4 PNK (NEB), 40 U ml −1 RNaseOUT (Thermo Scientific), 1 μl [ 32 P]ATP and 40 μl RNase-free water at 37°C for 1 h. The labeled probe was then purified by RNase-free micro bio-spin columns with Purification:Article Title: m 6 A Facilitates eIF4F-Independent mRNA Translation Article Snippet: The capped or non-capped mRNA (Hsp70-5'UTR) was synthesized using the mMessage mMachine T7 Ultra kit (Ambion). .. The probe was labeled in a 50ul reaction mixture containing 2 μl RNA probe (1 μM), 5 μl 10×T4 PNK buffer (NEB), 1 μl T4 PNK (NEB), 40 U ml −1 RNaseOUT (Thermo Scientific), 1 μl [ 32 P]ATP and 40 μl RNase-free water at 37°C for 1 h. The labeled probe was then purified by RNase-free micro bio-spin columns with |